IMBA Fly House
The Fly House provides research support for the growing number of scientists at IMBA working with the fruitfly Drosophila melanogaster as a genetic model system. Our service includes the generation of transgenic fly lines, gene targeting via homologous recombination to generate knock-out or knock-in mutants, large-scale in vivo RNAi screens, and the maintenance of stock collections.
One of the cornerstones of the Fly House is the generation of transgenic fly lines. We routinely inject DNA constructs into a range of commonly used host strains, including various landing site stocks for phiC31-mediated targeted integration, and subsequently perform all crosses to establish mapped and balanced transgenic stocks.
Although the use of homologous recombination to generate defined mutations is a well-established technique in several genetic model organisms, gene targeting in Drosophila has only recently been developed.
With the need to confirm the RNAi knock-down phenotypes with classical loss-of-function alleles or to tag genes at their endogenous locus, we have set up a Drosophila gene targeting service at the institute. Currently, we use an ends-out strategy of homologous recombination to create defined deletions or to add tag sequences to genes.
The first genome-wide collection of inducible RNAi lines, available through the Vienna Drosophila RNAi Center (VDRC), has revolutionized loss-of-function genetic screens in Drosophila. It is now possible to systematically investigate the function of each gene in a tissue of interest. We support scientists conducting large-scale in vivo RNAi screens as well as with subsequent follow up experiments to validate their hits.
Fly stock maintenance and plasmid collection
In addition to looking after lab stock collections, we keep various commonly used fly stocks such as balancers and virginizer lines. We also have a growing plasmid collection consisting mainly of vectors used for targeted integration or homologous recombination and work towards improving these tools.
Contact/Head of Department:
tel: +43-1-79044 - 4509
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