Publications

(2017)
 
Andersson-Rolf, A., Mustata, RC., Merenda, A., Kim, J., Perera, S., Grego, T., Andrews, K., Tremble, K., Silva, JC., Fink, J., Skarnes, WC., Koo, BK. (2017). One-step generation of conditional and reversible gene knockouts. Nat Methods. 14(3):287-289 (abstract)
 
Andersson-Rolf, A., Zilbauer, M., Koo, BK., Clevers, H. (2017). Stem Cells in Repair of Gastrointestinal Epithelia. Physiology (Bethesda). 32(4):278-289 (abstract)
 
Bartfeld, S., Koo, BK. (2017). Adult gastric stem cells and their niches. Wiley Interdiscip Rev Dev Biol. 6(2) (abstract)
 
Merenda, A., Andersson-Rolf, A., Mustata, RC., Li, T., Kim, H., Koo, BK. (2017). A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer. J Vis Exp. (125) (abstract)
 
Turco, MY., Gardner, L., Hughes, J., Cindrova-Davies, T., Gomez, MJ., Farrell, L., Hollinshead, M., Marsh, SGE., Brosens, JJ., Critchley, HO., Simons, BD., Hemberger, M., Koo, BK., Moffett, A., Burton, GJ. (2017). Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium. Nat Cell Biol. (abstract)
 
(2016)
 
Andersson-Rolf, A., Merenda, A., Mustata, RC., Li, T., Dietmann, S., Koo, BK. (2016). Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Dev Biol. 420(2):271-277 (abstract)
 
Andersson-Rolf A, Mustata RC, Merenda A, Perera S, Grego T, Kim J, Andrews K, Fink J, Skarnes WC, Koo BK. (2016). Rapid, one-step generation of biallelic conditional gene knockouts. BioRxiv.
 
Broutier, L., Andersson-Rolf, A., Hindley, CJ., Boj, SF., Clevers, H., Koo, BK., Huch, M. (2016). Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Nat Protoc. 11(9):1724-43 (abstract)
 
Fink, J., Koo, BK. (2016). Clonal Evolution of Stem Cells in the Gastrointestinal Tract. Adv Exp Med Biol. 908:11-25 (abstract)
 
Koo, BK., Huch, M. (2016). Organoids: A new in vitro model system for biomedical science and disease modelling and promising source for cell-based transplantation. Dev Biol. 420(2):197-198 (abstract)
 
(2015)
 
Andersson-Rolf A, Koo BK (2015). Stem cell-derived organoid cultures and genome editing tools. Cell Gene Therapy Insights. 1(2):243-251
 
Fink, J., Andersson-Rolf, A., Koo, BK. (2015). Adult stem cell lineage tracing and deep tissue imaging. BMB Rep. 48(12):655-67 (abstract)
 
Huch, M., Koo, BK. (2015). Modeling mouse and human development using organoid cultures. Development. 142(18):3113-25 (abstract)
 
Koo, BK., van Es, JH., van den Born, M., Clevers, H. (2015). Porcupine inhibitor suppresses paracrine Wnt-driven growth of Rnf43;Znrf3-mutant neoplasia. Proc Natl Acad Sci U S A. 112(24):7548-50 (abstract)
 
Schuijers, J., Junker, JP., Mokry, M., Hatzis, P., Koo, BK., Sasselli, V., van der Flier, LG., Cuppen, E., van Oudenaarden, A., Clevers, H. (2015). Ascl2 acts as an R-spondin/Wnt-responsive switch to control stemness in intestinal crypts. Cell Stem Cell. 16(2):158-70 (abstract)
 
(2014)
 
Andersson-Rolf, A., Fink, J., Mustata, RC., Koo, BK. (2014). A video protocol of retroviral infection in primary intestinal organoid culture. J Vis Exp. (90):e51765 (abstract)
 
Koo, BK., Clevers, H. (2014). Stem cells marked by the R-spondin receptor LGR5. Gastroenterology. 147(2):289-302 (abstract)
 
(2013)
 
Heijmans, J., van Lidth de Jeude, JF., Koo, BK., Rosekrans, SL., Wielenga, MC., van de Wetering, M., Ferrante, M., Lee, AS., Onderwater, JJ., Paton, JC., Paton, AW., Mommaas, AM., Kodach, LL., Hardwick, JC., Hommes, DW., Clevers, H., Muncan, V., van den Brink, GR. (2013). ER stress causes rapid loss of intestinal epithelial stemness through activation of the unfolded protein response. Cell Rep. 3(4):1128-39 (abstract)
 
Koo, BK., Sasselli, V., Clevers, H. (2013). Retroviral gene expression control in primary organoid cultures. Curr Protoc Stem Cell Biol. 27:Unit 5A.6. (abstract)
 
Schwank, G., Andersson-Rolf, A., Koo, BK., Sasaki, N., Clevers, H. (2013). Generation of BAC transgenic epithelial organoids. PLoS One. 8(10):e76871 (abstract)
 
Schwank, G., Koo, BK., Sasselli, V., Dekkers, JF., Heo, I., Demircan, T., Sasaki, N., Boymans, S., Cuppen, E., van der Ent, CK., Nieuwenhuis, EE., Beekman, JM., Clevers, H. (2013). Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. Cell Stem Cell. 13(6):653-8 (abstract)
 
Stange, DE., Koo, BK., Huch, M., Sibbel, G., Basak, O., Lyubimova, A., Kujala, P., Bartfeld, S., Koster, J., Geahlen, JH., Peters, PJ., van Es, JH., van de Wetering, M., Mills, JC., Clevers, H. (2013). Differentiated Troy+ chief cells act as reserve stem cells to generate all lineages of the stomach epithelium. Cell. 155(2):357-68 (abstract)
 
(2012)
 
Kim, HA., Koo, BK., Cho, JH., Kim, YY., Seong, J., Chang, HJ., Oh, YM., Stange, DE., Park, JG., Hwang, D., Kong, YY. (2012). Notch1 counteracts WNT/β-catenin signaling through chromatin modification in colorectal cancer. J Clin Invest. 122(9):3248-59 (abstract)
 
Koo, BK., Spit, M., Jordens, I., Low, TY., Stange, DE., van de Wetering, M., van Es, JH., Mohammed, S., Heck, AJ., Maurice, MM., Clevers, H. (2012). Tumour suppressor RNF43 is a stem-cell E3 ligase that induces endocytosis of Wnt receptors. Nature. 488(7413):665-9 (abstract)
 
Muñoz, J., Stange, DE., Schepers, AG., van de Wetering, M., Koo, BK., Itzkovitz, S., Volckmann, R., Kung, KS., Koster, J., Radulescu, S., Myant, K., Versteeg, R., Sansom, OJ., van Es, JH., Barker, N., van Oudenaarden, A., Mohammed, S., Heck, AJ., Clevers, H. (2012). The Lgr5 intestinal stem cell signature: robust expression of proposed quiescent '+4' cell markers. EMBO J. 31(14):3079-91 (abstract)
 
van Es, JH., Haegebarth, A., Kujala, P., Itzkovitz, S., Koo, BK., Boj, SF., Korving, J., van den Born, M., van Oudenaarden, A., Robine, S., Clevers, H. (2012). A critical role for the Wnt effector Tcf4 in adult intestinal homeostatic self-renewal. Mol Cell Biol. 32(10):1918-27 (abstract)
 
(2011)
 
de Lau, W., Barker, N., Low, TY., Koo, BK., Li, VS., Teunissen, H., Kujala, P., Haegebarth, A., Peters, PJ., van de Wetering, M., Stange, DE., van Es, JE., Guardavaccaro, D., Schasfoort, RB., Mohri, Y., Nishimori, K., Mohammed, S., Heck, AJ., Clevers, H. (2011). Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature. 476(7360):293-7 (abstract)
 
Kim, NS., Kim, HT., Kwon, MC., Choi, SW., Kim, YY., Yoon, KJ., Koo, BK., Kong, MP., Shin, J., Cho, Y., Kong, YY. (2011). Survival and differentiation of mammary epithelial cells in mammary gland development require nuclear retention of Id2 due to RANK signaling. Mol Cell Biol. 31(23):4775-88 (abstract)
 
Koo, BK., Stange, DE., Sato, T., Karthaus, W., Farin, HF., Huch, M., van Es, JH., Clevers, H. (2011). Controlled gene expression in primary Lgr5 organoid cultures. Nat Methods. 9(1):81-3 (abstract)
 
Min, JK., Park, H., Choi, HJ., Kim, Y., Pyun, BJ., Agrawal, V., Song, BW., Jeon, J., Maeng, YS., Rho, SS., Shim, S., Chai, JH., Koo, BK., Hong, HJ., Yun, CO., Choi, C., Kim, YM., Hwang, KC., Kwon, YG. (2011). The WNT antagonist Dickkopf2 promotes angiogenesis in rodent and human endothelial cells. J Clin Invest. 121(5):1882-93 (abstract)
 
(2010)
 
Stange, DE., Engel, F., Longerich, T., Koo, BK., Koch, M., Delhomme, N., Aigner, M., Toedt, G., Schirmacher, P., Lichter, P., Weitz, J., Radlwimmer, B. (2010). Expression of an ASCL2 related stem cell signature and IGF2 in colorectal cancer liver metastases with 11p15.5 gain. Gut. 59(9):1236-44 (abstract)
 
(2009)
 
Jeong, HW., Jeon, US., Koo, BK., Kim, WY., Im, SK., Shin, J., Cho, Y., Kim, J., Kong, YY. (2009). Inactivation of Notch signaling in the renal collecting duct causes nephrogenic diabetes insipidus in mice. J Clin Invest. 119(11):3290-300 (abstract)
 
Koo, BK., Lim, HS., Chang, HJ., Yoon, MJ., Choi, Y., Kong, MP., Kim, CH., Kim, JM., Park, JG., Kong, YY. (2009). Notch signaling promotes the generation of EphrinB1-positive intestinal epithelial cells. Gastroenterology. 137(1):145-55, 155.e1-3 (abstract)
 
Kwon, MC., Koo, BK., Kim, YY., Lee, SH., Kim, NS., Kim, JH., Kong, YY. (2009). Essential role of CR6-interacting factor 1 (Crif1) in E74-like factor 3 (ELF3)-mediated intestinal development. J Biol Chem. 284(48):33634-41 (abstract)
 
(2008)
 
Kim, YW., Koo, BK., Jeong, HW., Yoon, MJ., Song, R., Shin, J., Jeong, DC., Kim, SH., Kong, YY. (2008). Defective Notch activation in microenvironment leads to myeloproliferative disease. Blood. 112(12):4628-38 (abstract)
 
Kwon, MC., Koo, BK., Moon, JS., Kim, YY., Park, KC., Kim, NS., Kwon, MY., Kong, MP., Yoon, KJ., Im, SK., Ghim, J., Han, YM., Jang, SK., Shong, M., Kong, YY. (2008). Crif1 is a novel transcriptional coactivator of STAT3. EMBO J. 27(4):642-53 (abstract)
 
Lee, JH., Kim, TS., Yang, TH., Koo, BK., Oh, SP., Lee, KP., Oh, HJ., Lee, SH., Kong, YY., Kim, JM., Lim, DS. (2008). A crucial role of WW45 in developing epithelial tissues in the mouse. EMBO J. 27(8):1231-42 (abstract)
 
Song, R., Kim, YW., Koo, BK., Jeong, HW., Yoon, MJ., Yoon, KJ., Jun, DJ., Im, SK., Shin, J., Kong, MP., Kim, KT., Yoon, K., Kong, YY. (2008). Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development. J Exp Med. 205(11):2525-36 (abstract)
 
Yoon, KJ., Koo, BK., Im, SK., Jeong, HW., Ghim, J., Kwon, MC., Moon, JS., Miyata, T., Kong, YY. (2008). Mind bomb 1-expressing intermediate progenitors generate notch signaling to maintain radial glial cells. Neuron. 58(4):519-31 (abstract)
 
Yoon, MJ., Koo, BK., Song, R., Jeong, HW., Shin, J., Kim, YW., Kong, YY., Suh, PG. (2008). Mind bomb-1 is essential for intraembryonic hematopoiesis in the aortic endothelium and the subaortic patches. Mol Cell Biol. 28(15):4794-804 (abstract)
 
(2007)
 
Koo, BK., Yoon, MJ., Yoon, KJ., Im, SK., Kim, YY., Kim, CH., Suh, PG., Jan, YN., Kong, YY. (2007). An obligatory role of mind bomb-1 in notch signaling of mammalian development. PLoS One. 2(11):e1221 (abstract)
 
(2006)
 
Kim, NS., Kim, HJ., Koo, BK., Kwon, MC., Kim, YW., Cho, Y., Yokota, Y., Penninger, JM., Kong, YY. (2006). Receptor activator of NF-kappaB ligand regulates the proliferation of mammary epithelial cells via Id2. Mol Cell Biol. 26(3):1002-13 (abstract)
 
Song, R., Koo, BK., Yoon, KJ., Yoon, MJ., Yoo, KW., Kim, HT., Oh, HJ., Kim, YY., Han, JK., Kim, CH., Kong, YY. (2006). Neuralized-2 regulates a Notch ligand in cooperation with Mind bomb-1. J Biol Chem. 281(47):36391-400 (abstract)
 
Yoo, KW., Kim, EH., Jung, SH., Rhee, M., Koo, BK., Yoon, KJ., Kong, YY., Kim, CH. (2006). Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish. FEBS Lett. 580(18):4409-16 (abstract)
 
(2005)
 
Koo, BK., Lim, HS., Song, R., Yoon, MJ., Yoon, KJ., Moon, JS., Kim, YW., Kwon, MC., Yoo, KW., Kong, MP., Lee, J., Chitnis, AB., Kim, CH., Kong, YY. (2005). Mind bomb 1 is essential for generating functional Notch ligands to activate Notch. Development. 132(15):3459-70 (abstract)
 
Koo, BK., Yoon, KJ., Yoo, KW., Lim, HS., Song, R., So, JH., Kim, CH., Kong, YY. (2005). Mind bomb-2 is an E3 ligase for Notch ligand. J Biol Chem. 280(23):22335-42 (abstract)
 

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